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Calculate serial dilution factors instantly with our free online Serial Dilution Factor Calculator. Accurate, easy-to-use, and perfect for lab and classroom use.
If you’re working in a lab, classroom, or research setting, understanding serial dilution is essential. It’s one of the most common techniques used in biology, chemistry, and medicine. That’s why we’ve built an easy-to-use Serial Dilution Factor Calculator to help you perform dilution calculations with 100% accuracy, fast and effortlessly.
This tool helps you quickly calculate dilution factors, solvent volume needed, and even track multiple-step dilution series without any manual math.
A serial dilution is a step-by-step method of diluting a solution multiple times, where the output from one dilution becomes the input for the next. It is most commonly used to reduce a dense culture of cells or molecules to a more usable concentration.
For example, a 1:10 dilution repeated three times results in a final dilution of 1:1000. This is because each step dilutes the solution 10 times more, and it compounds as:
Total Dilution Factor = Step Dilution Factor ^ Number of Steps
Total DF = 10 ^ 3 = 1000
To calculate a serial dilution, you need to know:
The formula is simple:
Total Dilution Factor = (Step Dilution Factor) ^ (Number of Steps)
If you're using a constant dilution factor (like 1:5) across 4 steps:
Total DF = 5 ^ 4 = 625
Our dilution series calculator automates this, letting you enter your step dilution and number of steps to instantly calculate the total dilution factor, volume to transfer in each step, and how much solvent to add.
The dilution factor (DF) is the ratio of the final volume to the volume of the solute or concentrated solution you are diluting.
Dilution Factor = Final Volume / Solute Volume
For example, if you mix 1 mL of a solution into 9 mL of water, your total volume is 10 mL:
DF = 10 / 1 = 10
That’s a 1:10 dilution.
A 20X dilution means the solution is 20 times more concentrated than needed. To dilute it to a working solution:
C1V1 = C2V2
Using the formula:
So if you want 100 mL of a 1X solution from a 20X stock:
V1 = (1 × 100) / 20 = 5 mL
Add 5 mL of 20X stock to 95 mL of solvent (water or buffer) to get 100 mL of 1X working solution.
Using our calculator is easy:
You can select Single Dilution, Serial Dilution, or Concentration Calculator tab based on your need.
For serial dilution:
You’ll get:
The results are clear and formatted for both beginners and experts.
Whether you're a student, lab technician, or scientist, our Serial Dilution Factor Calculator helps you avoid manual mistakes and speeds up your workflow. It’s designed for clarity, precision, and ease of use. Plus, it supports multiple units and even calculates concentration changes using the C1V1 = C2V2 formula.
So next time you're setting up your dilution series, let our calculator handle the math for you.
Serial dilution is commonly used in microbiology, chemistry, and medical labs to reduce concentration step-by-step for counting cells, preparing standards, or titrating compounds.
Use the formula:
DF = Final Volume / Solute Volume
For serial:
Total DF = (Step DF) ^ Number of Steps
It means the original solution has been diluted 1000 times. For example, 1 mL of solute in 999 mL of solvent.
Yes! It supports mL, μL, L, nL, and pL. It also handles concentration units like M, mM, μM, etc.
This is used to calculate concentrations and volumes:
You can rearrange the formula to find any unknown.